Phytochemical Screening and Preliminary Evaluation of Bioactive Constituents in Methanolic Leaf Extract of Datura alba

Abstract

The present observe specializes in the phytochemical screening and preliminary assessment of bioactive ingredients inside the methanolic leaf extract of Datura alba. The fresh leaves had been amassed, shade-dried, powdered, and macerated in 90% methanol to gain the crude extract. Qualitative phytochemical exams found out the presence of a diverse variety of secondary metabolites consisting of saponins, phenols, alkaloids, flavonoids, terpenoids, glycosides, proteins, and phytosterols, at the same time as phlobatannins had been extensively absent. High-overall performance liquid chromatography (HPLC) evaluation identified three most important peaks inside a retention time range of 0 to six minutes, with two major compounds constituting over ninety nine% of the extract. These findings reveal that Datura alba harbors a wealthy phytochemical profile that supports its traditional medicinal makes use of and capability therapeutic programs. The examine underscores the need for in addition bioactivity-guided isolation and pharmacological critiques of its bioactive compounds.